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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in many tumors, which leads to chromosome instability, and therefore may well lead to tumorigenesis. This protein was located to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant job in endorsing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants have been reported but specific nucleotide sequence info is not really but available.

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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide normally incorporates the epitope recognized through the antibody.

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This guidebook outlines even further possible optimization of cellular and immunoassay parameters to be sure the very best success are...

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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the best possible final results are...

Antibodies bound to the blocking peptide no longer bind to the epitope over the goal protein. By evaluating the staining through the blocked antibody compared to the antibody alone, you can see which staining is distinct.

Goal Information Cyclin E belongs into the hugely conserved cyclin loved ones, whose members are characterised by a remarkable periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression click here and degradation patterns which add towards the temporal coordination of each and every mitotic occasion. Cyclin E varieties a complex with and features as a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S section boundary and it is degraded as cells development as a result of S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...

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